An anti-CRISPR that pulls apart a CRISPR–Cas complex – Nature

-


  • Mayo-Munoz, D., Pinilla-Redondo, R., Camara-Wilpert, S., Birkholz, N. & Fineran, P. C. Inhibitors of bacterial immune systems: discovery, mechanisms and applications. Nat. Rev. Genet. 25, 237–254 (2024).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Wiegand, T., Karambelkar, S., Bondy-Denomy, J. & Wiedenheft, B. Structures and strategies of anti-CRISPR-mediated immune suppression. Ann. Rev. Microbiol. 74, 21–37 (2020).

    Article 
    CAS 

    Google Scholar
     

  • Davidson, A. R. et al. Anti-CRISPRs: protein inhibitors of CRISPR-Cas systems. Annu. Rev. Biochem. 89, 309–332 (2020).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Bondy-Denomy, J. et al. A unified resource for tracking anti-CRISPR names. CRISPR J. 1, 304–305 (2018).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Makarova, K. S. et al. Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants. Nat. Rev. Microbiol. 18, 67–83 (2020).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Barrangou, R. & Marraffini, L. A. CRISPR-Cas systems: prokaryotes upgrade to adaptive immunity. Mol. Cell 54, 234–244 (2014).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Pawluk, A., Davidson, A. R. & Maxwell, K. L. Anti-CRISPR: discovery, mechanism and function. Nat. Rev. Microbiol. 16, 12–17 (2018).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Yin, P., Zhang, Y., Yang, L. & Feng, Y. Non-canonical inhibition strategies and structural basis of anti-CRISPR proteins targeting type I CRISPR-Cas systems. J. Mol. Biol. 435, 167996 (2023).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Wiedenheft, B. et al. RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions. Proc. Natl Acad. Sci. USA 108, 10092–10097 (2011).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Chowdhury, S. et al. Structure reveals mechanisms of viral suppressors that intercept a CRISPR RNA-guided surveillance complex. Cell 169, 47–57 (2017).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Guo, T. W. et al. Cryo-EM structures reveal mechanism and inhibition of DNA targeting by a CRISPR-Cas surveillance complex. Cell 171, 414–426 (2017).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Zhang, K. et al. Inhibition mechanisms of AcrF9, AcrF8, and AcrF6 against type I-F CRISPR-Cas complex revealed by cryo-EM. Proc. Natl Acad. Sci. USA 117, 7176–7182 (2020).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Gabel, C., Li, Z., Zhang, H. & Chang, L. Structural basis for inhibition of the type I-F CRISPR-Cas surveillance complex by AcrIF4, AcrIF7 and AcrIF14. Nucleic Acids Res. 49, 584–594 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Pawluk, A. et al. Inactivation of CRISPR-Cas systems by anti-CRISPR proteins in diverse bacterial species. Nat. Microbiol. 1, 16085 (2016).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Leon, L. M., Park, A. E., Borges, A. L., Zhang, J. Y. & Bondy-Denomy, J. Mobile element warfare via CRISPR and anti-CRISPR in Pseudomonas aeruginosa. Nucleic Acids Res. 49, 2114–2125 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Lee, S. Y., Birkholz, N., Fineran, P. C. & Park, H. H. Molecular basis of anti-CRISPR operon repression by Aca10. Nucleic Acids Res. 50, 8919–8928 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Bondy-Denomy, J., Pawluk, A., Maxwell, K. L. & Davidson, A. R. Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system. Nature 493, 429–432 (2013).

    Article 
    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Soding, J., Biegert, A. & Lupas, A. N. The HHpred interactive server for protein homology detection and structure prediction. Nucleic Acids Res. 33, W244–W248 (2005).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Bondy-Denomy, J. et al. Multiple mechanisms for CRISPR-Cas inhibition by anti-CRISPR proteins. Nature 526, 136–139 (2015).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Schreiter, E. R. & Drennan, C. L. Ribbon-helix-helix transcription factors: variations on a theme. Nat. Rev. Microbiol. 5, 710–720 (2007).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Morgan, G. J., Hatfull, G. F., Casjens, S. & Hendrix, R. W. Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus. J. Mol. Biol. 317, 337–359 (2002).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Sousa, R. Structural mechanisms of chaperone mediated protein disaggregation. Front. Mol. Biosci. 1, 12 (2014).

    Article 
    ADS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Olivares, A. O., Baker, T. A. & Sauer, R. T. Mechanistic insights into bacterial AAA+ proteases and protein-remodelling machines. Nat. Rev. Microbiol. 14, 33–44 (2016).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Haurwitz, R. E., Sternberg, S. H. & Doudna, J. A. Csy4 relies on an unusual catalytic dyad to position and cleave CRISPR RNA. EMBO J. 31, 2824–2832 (2012).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Altschul, S. F. & Koonin, E. V. Iterated profile searches with PSI-BLAST-a tool for discovery in protein databases. Trends Biochem. Sci. 23, 444–447 (1998).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Chivers, P. T. & Sauer, R. T. NikR is a ribbon-helix-helix DNA-binding protein. Protein Sci. 8, 2494–2500 (1999).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Gibson, D. G. et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods 6, 343–345 (2009).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Harrington, L. B. et al. A broad-spectrum inhibitor of CRISPR-Cas9. Cell 170, 1224–1233 (2017).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • van den Ent, F. & Lowe, J. RF cloning: a restriction-free method for inserting target genes into plasmids. J. Biochem. Biophys. Methods 67, 67–74 (2006).

    Article 
    PubMed 

    Google Scholar
     

  • Howe, M. M. Prophage deletion mapping of bacteriophage Mu-1. Virology 54, 93–101 (1973).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Cady, K. C., Bondy-Denomy, J., Heussler, G. E., Davidson, A. R. & O’Toole, G. A. The CRISPR/Cas adaptive immune system of Pseudomonas aeruginosa mediates resistance to naturally occurring and engineered phages. J. Bacteriol. 194, 5728–5738 (2012).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Lee, D. G. et al. Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial. Genome Biol. 7, R90 (2006).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Cady, K. C. & O’Toole, G. A. Non-identity-mediated CRISPR-bacteriophage interaction mediated via the Csy and Cas3 proteins. J. Bacteriol. 193, 3433–3445 (2011).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Garcia, B. et al. Anti-CRISPR AcrIIA5 potently inhibits all Cas9 homologs used for genome editing. Cell Rep. 29, 1739–1746 (2019).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Otwinowski, Z. & Minor, W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 276, 307–326 (1997).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • McCoy, A. J. et al. Phaser crystallographic software. J. Appl. Crystallogr. 40, 658–674 (2007).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. D 66, 486–501 (2010).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Liebschner, D. et al. Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix. Acta Crystallogr. D 75, 861–877 (2019).

    Article 
    ADS 
    CAS 

    Google Scholar
     

  • Adams, P. D. et al. PHENIX: building new software for automated crystallographic structure determination. Acta Crystallogr. D 58, 1948–1954 (2002).

    Article 
    ADS 
    PubMed 

    Google Scholar
     

  • Pawluk, A. et al. Naturally occurring off-switches for CRISPR-Cas9. Cell 167, 1829–1838 (2016).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Lu, W. T., Trost, C. N., Muller-Esparza, H., Randau, L. & Davidson, A. R. Anti-CRISPR AcrIF9 functions by inducing the CRISPR-Cas complex to bind DNA non-specifically. Nucleic Acids Res. 49, 3381–3393 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Nogues, M. V., Vilanova, M. & Cuchillo, C. M. Bovine pancreatic ribonuclease A as a model of an enzyme with multiple substrate binding sites. Biochim. Biophys. Acta 1253, 16–24 (1995).

    Article 
    PubMed 

    Google Scholar
     



  • Source link

    Latest news

    Here’s What We Know About DOGE 2.0

    Leah Feiger: When we come back, we'll share our recommendations for what to check out on WIRED.com this...

    Startups Weekly: Still running | Tech Zone Daily

    Welcome to Startups Weekly — your weekly recap of everything you can’t miss from the world of startups....

    Metadata Shows the FBI’s ‘Raw’ Jeffrey Epstein Prison Video Was Likely Modified

    The United States Department of Justice this week released nearly 11 hours of what it described as “full...

    Prime Day Deals on WIRED’s Top Air Fryer and Espresso Machine

    Amazon Prime Day sales are timed for early summer—the time of enjoyment. The time you spoil yourself. Most...

    Julie Wainwright joins Tech Zone Daily Disrupt 2025 in a fireside chat

    Tech Zone Daily Disrupt 2025 returns to Moscone West in San Francisco from October 27–29, uniting over 10,000+...

    Coffee! Coffee Now! Get Your Caffeine Fix With These Prime Day Deals

    What’s more WIRED than coffee? Before you plug into the matrix, you need your coffee fix. We know...

    Must read

    You might also likeRELATED
    Recommended to you