A pore-forming antiphage defence is activated by oligomeric phage proteins – Nature

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  • Payne, L., Jackson, S. & Pinilla-Redondo, R. Supramolecular assemblies in bacterial immunity: an emerging paradigm. Trends Microbiol. 32, 828–831 (2024).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Lau, R. K. et al. Structure and mechanism of a cyclic trinucleotide-activated bacterial endonuclease mediating bacteriophage immunity. Mol. Cell 77, 723–733 (2020).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Duncan-Lowey, B., McNamara-Bordewick, N. K., Tal, N., Sorek, R. & Kranzusch, P. J. Effector-mediated membrane disruption controls cell death in CBASS antiphage defense. Mol. Cell 81, 5039–5051 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Ofir, G. et al. DISARM is a widespread bacterial defence system with broad anti-phage activities. Nat. Microbiol. 3, 90–98 (2018).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Rousset, F. et al. Phages and their satellites encode hotspots of antiviral systems. Cell Host Microbe 30, 740–753 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Burman, N. et al. A virally encoded tRNA neutralizes the PARIS antiviral defence system. Nature 634, 424–431 (2024).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Kawai, T. & Akira, S. The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nat. Immunol. 11, 373–384 (2010).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Takeuchi, O. & Akira, S. Pattern recognition receptors and inflammation. Cell 140, 805–820 (2010).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Iwasaki, A. A virological view of innate immune recognition. Annu. Rev. Microbiol. 66, 177–196 (2012).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Kayagaki, N. et al. Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling. Nature 526, 666–671 (2015).

    Article 
    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Gao, L. et al. Diverse enzymatic activities mediate antiviral immunity in prokaryotes. Science 369, 1077–1084 (2020).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Hu, H. et al. Structure and mechanism of the Zorya anti-phage defence system. Nature 639, 1093–1101 (2025).

    Article 
    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Zhang, Z. et al. Kiwa is a membrane-embedded defense supercomplex activated at phage attachment sites. Cell 188, 5862–5877.e23 (2025).

  • Johnson, A. G. et al. Bacterial gasdermins reveal an ancient mechanism of cell death. Science 375, 221–225 (2022).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Wein, T. et al. CARD domains mediate anti-phage defence in bacterial gasdermin systems. Nature 639, 727–734 (2025).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Tsao, Y.-F. et al. Phage morons play an important role in Pseudomonas aeruginosa phenotypes. J. Bacteriol. 200, e00189-18 (2018).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Patel, P. H. et al. Anti-phage defence through inhibition of virion assembly. Nat. Commun. 15, 1644 (2024).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Cahill, J. et al. Spatial and temporal control of lysis by the lambda holin. mBio 15, e01290-23 (2023).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Rojas, E. R. et al. The outer membrane is an essential load-bearing element in Gram-negative bacteria. Nature 559, 617–621 (2018).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Whatmore, A. M. & Reed, R. H. Determination of turgor pressure in Bacillus subtilis: a possible role for K+ in turgor regulation. J. Gen. Microbiol. 136, 2521–2526 (1990).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Dedeo, C. L., Cingolani, G. & Teschke, C. M. Portal protein: the orchestrator of capsid assembly for the dsDNA tailed bacteriophages and herpesviruses. Annu. Rev. Virol. 6, 141–160 (2019).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Maffei, E. et al. Systematic exploration of Escherichia coli phage–host interactions with the BASEL phage collection. PLoS Biol. 19, e3001424 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Kondabagil, K. R. & Rao, V. B. A critical coiled coil motif in the small terminase, gp16, from bacteriophage T4: insights into DNA packaging initiation and assembly of packaging motor. J. Mol. Biol. 358, 67–82 (2006).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Sun, S. et al. Structure and function of the small terminase component of the DNA packaging machine in T4-like bacteriophages. Proc. Natl Acad. Sci. USA 109, 817–822 (2012).

    Article 
    ADS 
    PubMed 

    Google Scholar
     

  • Leffers, G. & Rao, V. B. Biochemical characterization of an ATPase activity associated with the large packaging subunit gp17 from bacteriophage T4*. J. Biol. Chem. 275, 37127–37136 (2000).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Ashkenazy, H. et al. ConSurf 2016: an improved methodology to estimate and visualize evolutionary conservation in macromolecules. Nucleic Acids Res. 44, W344–W350 (2016).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Tanaka, K., Caaveiro, J. M. M., Morante, K., González-Mañas, J. M. & Tsumoto, K. Structural basis for self-assembly of a cytolytic pore lined by protein and lipid. Nat. Commun. 6, 6337 (2015).

    Article 
    ADS 
    PubMed 

    Google Scholar
     

  • Rath, A. & Deber, C. M. Surface recognition elements of membrane protein oligomerization. Proteins Struct. Funct. Bioinf. 70, 786–793 (2008).

    Article 
    CAS 

    Google Scholar
     

  • Parker, M. W. & Feil, S. C. Pore-forming protein toxins: from structure to function. Prog. Biophys. Mol. Biol. 88, 91–142 (2005).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Mueller, M., Grauschopf, U., Maier, T., Glockshuber, R. & Ban, N. The structure of a cytolytic α-helical toxin pore reveals its assembly mechanism. Nature 459, 726–730 (2009).

    Article 
    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Wiener, M., Freymann, D., Ghosh, P. & Stroud, R. M. Crystal structure of colicin Ia. Nature 385, 461–464 (1997).

    Article 
    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Stone, T. A. et al. Positive charge patterning and hydrophobicity of membrane-active antimicrobial peptides as determinants of activity, toxicity, and pharmacokinetic stability. J. Med. Chem. 62, 6276–6286 (2019).

    Article 
    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Tal, N. et al. Cyclic CMP and cyclic UMP mediate bacterial immunity against phages. Cell 184, 5728–5739 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • VanderWal, A. R. et al. Csx28 is a membrane pore that enhances CRISPR-Cas13b–dependent antiphage defense. Science 380, 410–415 (2023).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Ganser-Pornillos, B. K. & Pornillos, O. Restriction of HIV-1 and other retroviruses by TRIM5. Nat. Rev. Microbiol. 17, 546–556 (2019).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Gao, L. A. et al. Prokaryotic innate immunity through pattern recognition of conserved viral proteins. Science 377, eabm4096 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Ojobor, C. D. The Noncontractile Phage Tail-like Bacterial Killing Nanomachines—Characterizing the Specificity Determinants of the F-Pyocins of Pseudomonas aeruginosa. PhD thesis, Univ. of Toronto (2022).

  • Hmelo, L. R. et al. Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange. Nat. Protoc. 10, 1820–1841 (2015).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Stokar-Avihail, A. et al. Discovery of phage determinants that confer sensitivity to bacterial immune systems. Cell 186, 1863–1876 (2023).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Jakočiūnė, D. & Moodley, A. A rapid bacteriophage DNA extraction method. Methods Protoc. 1, 27 (2018).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Punjani, A., Rubinstein, J. L., Fleet, D. J. & Brubaker, M. A. CryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. Nat. Methods 14, 290–296 (2017).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Bepler, T. et al. Positive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs. Nat. Methods 16, 1153–1160 (2019).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. D 66, 486–501 (2010).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Croll, T. I. ISOLDE: a physically realistic environment for model building into low-resolution electron-density maps. Acta Crystallogr. D 74, 519–530 (2018).

    Article 
    ADS 
    CAS 

    Google Scholar
     

  • Liebschner, D. et al. Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix. Acta Crystallogr. D 75, 861–877 (2019).

    Article 
    ADS 
    CAS 

    Google Scholar
     

  • Abramson, J. et al. Accurate structure prediction of biomolecular interactions with AlphaFold 3. Nature 630, 493–500 (2024).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Jumper, J. et al. Highly accurate protein structure prediction with AlphaFold. Nature 596, 583–589 (2021).

    Article 
    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Pettersen, E. F. et al. UCSF ChimeraX: structure visualization for researchers, educators, and developers. Protein Sci. 30, 70–82 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar
     

  • Harrington, L. B. et al. A broad-spectrum inhibitor of CRISPR-Cas9. Cell 170, 1224–1233 (2017).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Zeng, F. et al. A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends. BMC Biotech. 17, 32 (2017).

    Article 

    Google Scholar
     

  • Altschul, S. F. et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402 (1997).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Bouras, G. et al. Pharokka: a fast scalable bacteriophage annotation tool. Bioinformatics 39, btac776 (2023).

    Article 
    CAS 
    PubMed 

    Google Scholar
     



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